Ph.D.
Professor
M.S. Swaminathan School of Agriculture
 
Department: Biotechnology
Phone: 9178000571
 

About Me

Professional summary
Over 13 years of multidisciplinary professional specializations including teaching and research experience in field of Biotechnology and Agricultural Sciences. Teaching experience in Biotechnology to post-graduate and undergraduate students with over eight years experience in screening and evaluation of germ-plasm varieties, allele mining of genes conferring salt tolerance, identification and validation of differentially expressed genes at National Institutes i.e., Indian Institute of Rice Research (IIRR)/Directorate of Rice Research (DRR), Hyderabad and National Research Centre of Plant Biotechnology (NRCPB), Indian Agricultural Research Institute (IARI), New Delhi. I possess a sound knowledge of PCR-based allele mining, qRT PCR, scanning electron microscope (SEM), drought screening techniques, physiological/biochemical analysis and microarray techniques with demonstrated ability to use statistical analysis and well versed with different Bioinformatics techniques, molecular breeding techniques and statistical analysis tools. I possess the ability to work independently as well as in a team with strong interpersonal relationship and leadership skills. 

I was an invited �Visiting Faculty� under a research program on �Detection of freshness among cereals crops using fluorescence spectroscopy to determine the fluorescence level based on the presence of fluorescent components� at Graduate School of Agriculture, Kyoto University, Kyoto, Japan for a period of three months from 30th November 2015 to 29th February 2016. I was felicitated with �Scientist Associate Award� by Society for Scientific Development in Agriculture and Technology (SSDAT) at National Conference on Emerging Challenges and Opportunities in Biotic and Abiotic Stress Management (ECOBASM - 2014), 13th and 14th December 2014 at Directorate of Rice Research, Hyderabad. I was an �Invited Young Scientist with IRRI-PHILIPPINES Sponsorship� to attend and present a part of my doctoral research work entitled �Whole Transcriptomic (RNA) Sequencing and Leaf Photosynthetic Response to Salinity in High Yielding Rice Introgression Lines Derived from KMR3 X O. rufipogon�, at 4th International Rice Congress (IRC2014) between 27th and 31st October 2014 at Bangkok, Thailand.
 
Research Fields

  • Plant Biotechnology and Molecular Biology: Plant DNA and RNA extraction
  • cDNA synthesis
  • polymerase chain reaction (PCR) amplification
  • validation of differentially expressed genes using qRT-PCR (ABI and Stratagen). Microarray analysis and cloning of genes tolerant to salt stress in rice introgression lines. Scanning electron microscopy (SEM) of plant samples by using QuantomiX WET-SEM capsules. Back cross breeding
  • plant tissue culture
  • micropropagation
  • callus and somatic cell induction both by leaf and stem inoculation
  • Flame photometry for ion analysis
  • Microbiology: Biochemical tests
  • Screening
  • Maintenance of pure cultures of bacteria
  • fungi and its preservation
  • Fermentation and Mushroom cultivation. Seri- Biotechnology: Silkworm rearing and breeding techniques
  • Trace element quantification by using Atomic Absorption Spectrophotometer
  • Protein quantification of membrane proteins both by Spectrophotometric and Gel electrophoretic method � Native and SDS-PAGE electrophoresis
  • DNA extraction
  • quantification and amplification from silkworm moth tissues. Computer/Bioinformatics: knowledge of bioinformatics like NCBI- pubMed
  • BLAST
  • phi BLAST
  • psi BLAST
  • FASTA
  • skilled knowledge of MS Office and soft-wares for the prediction of identification of cis-acting elements viz.
  • PLANT CARE
  • PLACE
  • JASPAR
  • etc. and skills to predict miRNA in in silico.
Teaching

  • Plant Physiology
  • Plant Biotechnology
  • Computational Biology
  • Biotechnology for Crop Improvement

Expertise

  • Plant DNA and RNA extraction
  • cDNA synthesis
  • polymerase chain reaction (PCR) amplification
  • validation of differentially expressed genes using qRT-PCR (ABI and Stratagen). Microarray analysis and cloning of genes tolerant to salt stress in rice introgression lines. Scanning electron microscopy (SEM) of plant samples by using QuantomiX WET-SEM capsules. Back cross breeding
  • plant tissue culture
  • micropropagation
  • callus and somatic cell induction both by leaf and stem inoculation
  • Flame photometry for ion analysis. Seri- Biotechnology: Silkworm rearing and breeding techniques
  • Trace element quantification by using Atomic Absorption Spectrophotometer
  • Protein quantification of membrane proteins both by Spectrophotometric and Gel electrophoretic method
  • Native and SDS-PAGE electrophoresis
  • DNA extraction
  • quantification and amplification from silkworm moth tissues. Computer/Bioinformatics: knowledge of bioinformatics like NCBI- pubMed
  • BLAST
  • phi BLAST
  • psi BLAST
  • FASTA
  • skilled knowledge of MS Office and soft-wares for the prediction of identification of cis-acting elements viz.
  • PLANT CARE
  • PLACE
  • JASPAR
  • etc. and skills to predict miRNA in in silico.

Interests

  • Plant Physiology and Plant Biotechnology